![]() These include Assay of Transposase Accessible Chromatin sequencing (ATAC-seq), DNase I hypersensitive sites sequencing (DNase-seq) and Formaldehyde-Assisted Isolation of Regulatory Elements sequencing (FAIRE-seq), all of which interrogate chromatin accessibility Chromatin Immuno-Precipitation sequencing (ChIP-seq) which measures transcription factor (TF) binding and histone modifications and Micrococcal Nuclease sequencing (MNase-seq) which detects nucleosome positioning and occupancy. Recent gene regulation studies have focused on epigenetics, and through the advances of high-throughput sequencing technologies, various assays have been developed to decipher the epigenetic landscape. All three scales of DNA condensation and their interplay contribute to gene regulation. Chromatin can dynamically switch between transcriptionally active euchromatin and inactive heterochromatin. ![]() ![]() Mammalian DNA is highly condensed through three major hierarchical scales the first is the nucleosome which then wraps into chromatin leading to the third hierarchy, the chromosome. ![]()
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